Rabbit Anti-ND5 (MT-ND5, MTND5, NADH5, ND5, NADH-ubiquinone oxidoreductase chain 5, NADH dehydrogenase subunit 5)
NADH-ubiquinone oxidoreductase chain 5, EC=1.6.5.3, NADH dehydrogenase subunit 5, MTND5, NADH5
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.Anderson S., Nature 290:457-465(1981). Horai S., Proc. Natl. Acad. Sci. U.S.A. 92:532-536(1995). Maca-Meyer N., BMC Genet. 2:13-13(2001).
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:3000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to internal of human MT-ND5.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
