Rabbit Anti-TGM2 (TGase-2, Transglutaminase-2, TGC, Protein-glutamine gamma-glutamyltransferase 2, Tissue Transglutaminase, Transglutaminase C, TG(C), TGase C, Transglutaminase H, TGase H)
Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1000-1:2000 Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein of human TGM2
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
