Mouse Anti-14-3-3 epsilon
Tyrosine 3-monooxygenase/Tryptophan 5-monooxygenase Activation Protein epsilon Polypeptide, YWHAE, MDS, MDCR, KCIP-1, 14-3-3 epsilon, Tyrosine 3/Tryptophan 5 -monooxygenase Activation Protein, epsilon Polypeptide, Tyrosine 3-monooxygenase/Tryptophan 5-monooxygenase Activation Protein, epsilon Polypeptide 14 3 3 E, 14 3 3E, KCIP 1, KCIP1, Mitochondrial import stimulation Factor L subunit, Protein Kinase C Inhibitor Protein1, Tyrosine 3/Tryptophan 5 monooxygenase Activation Protein epsilon Polypeptide
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms,beta, gamma,epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-2000 Optimal dilutions to be determined by the researcher.
Hybridoma
FO myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa1-255 from human 14-3-3 epsilon expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Purity
Purified by Protein G affinity chromatography.|
Specificity
Recognizes human 14-3-3 epsilon.
