Technical Data

351230
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b,k
Grade
Affinity Purified
Applications
E IC IF WB
Crossreactivity
Hu
Accession #
NP_003893
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-FUBP1 (Far upstream element (FuSE) Binding Protein 1, FBP, FuBP)

FUBP1 is a transcriptional regulator and fulfills an important function in the precise control of c-myc transcription. The c-Myc protein is a transcription factor which regulates the transcription of many different target genes that play a role in proliferation, cell cycle progression, differentiation, apoptosis and cell metabolism. Consequently, FUBP1 is also involved in the regulation of proliferation and differentiation, as confirmed by different experimental approaches. Knockdown of FUBP1 or expression of a dominant-negative FUBP1 (DNA-binding domain lacking effector activity) led to proliferation arrest in U2OS and Saos-2 osteosarcoma cells due to reduced c-myc expression.

Applications
Suitable for use in ELISA, Western Blot, Immunocytochemistry/Immunofluorescence. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
F0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa279-44 from human FUBP1 expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human FUBP1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Duncan R., et al. (1994) Genes Dev. 8(4): 465-480, 2. Liu J., et al. (2006) EMBO J. 25(10): 2119-2130.
USBio References
No references available
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