Technical Data

351878
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
D1K6D
Grade
Purified
Applications
IP WB
Crossreactivity
Hu
Gene ID
10135
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PBEF (Nicotinamide Phosphoribosyltransferase, NAMPT, Pre-B Cell-enhancing Factor, Visfatin)

Nicotinamide phosphoribosyltransferase (NAMPT; also known as Pre-B cell-enhancing factor PBEF) catalyzes the synthesis of nicotinamide mononucleotide (NMN) from nicotinamide and 5-phosphoribosylpyrophosphate (PRPP), the rate-limiting step in the NAD biosynthesis pathway starting from nicotinamide (1,2). NAD biosynthesis mediated by NAMPT plays a critical role in glucose-stimulated insulin secretion in pancreatic beta cells (3). Both NAMPT inhibitors and activators have been sought for clinical applications (4,5). NAMPT has intra- and extracellular forms (iNAMPT and eNAMPT), and deacetylation of iNAMPT by SIRT1 promotes eNAMPT secretion through a nonclassical secretory pathway (3,6). eNAMPT, independent of its enzymatic activity, can induce epithelial-to-mesenchymal transition in mammary epithelial cells and promote monocyte differentiation into tumor-supporting M2 macrophage (7,8).

Applications
Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Gly122 of human PBEF/NAMPT.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of total human PBEF/NAMPT.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Imai, S. (2009) Curr Pharm Des 15, 20-8. 2. Samal, B. et al. (1994) Mol Cell Biol 14, 1431-7. 3. Revollo, J.R. et al. (2007) Cell Metab 6, 363-75. 4. Montecucco, F. et al. (2013) Curr Drug Targets 14, 637-43. 5. Wang, G. et al. (2014) Cell 158, 1324-34. 6. Yoon, M.J. et al. (2015) Cell Metab 21, 706-17. 7. Soncini, D. et al. (2014) J Biol Chem 289, 34189-204. 8. Audrito, V. et al. (2015) Blood 125, 111-23
USBio References
No references available
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