352098
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IC IF WBCrossreactivity
Hu MoAccession #
NP_689619Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-AFAP1L, NT (Actin Filament-Associated Protein 1-like 1, AFAP1-like Protein)
The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation. Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.
Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 20ug/ml Western Blot: 1ug/ml Immunocytochemistry: 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
A549 Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 19aa from human AFAP1L at N-terminal.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human AFAP1L. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Snyder BN, Cho Y, Qian Y, et al. AFAP1L1 is a novel adaptor protein of the AFAP family that interacts with cortactin and localizes to invadosomes. Eur. J. Cell Biol. 2011; 90:376-89 2. Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament- associated pp60src substrate. Mol. Cell. Biol. 1993; 13:7892-900. 3. Baisden JM, Gatesman AS, Cherezova L, et al. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene 2001; 20:6607-16 4. Saltel F, Daubon T, Juin A, et al. Invadosomes: intriguing structures with promise. Eur. J. Cell Biol. 2011; 90:100-7.USBio References
No references available