Rabbit Anti-DENR (DRP, DRP1, SMAP-3, H14, Density-regulated protein, Protein DRP1)
DENR (density-regulated protein), also designated DRP or smooth muscle cell associated protein 3 (SMAP-3), is a 198 amino acid protein that may be involved in the translation of target mRNAs by scanning and recognition of the initiation codon (1). It plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1 (2,3). DENR contains a SUI1 domain and is found in a variety of tissues with highest levels present in skeletal and cardiac muscle. It is up-regulated in ovarian and breast cancer cells by ERBB2 overexpression (4).
Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 20ug/ml Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Brain Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to 18aa near the center of human DENR.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DENR. Species Crossreactivity: mouse and rat
