Technical Data

352339
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Accession #
NP_004908
Gene #
KLK4
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-KLK4 (ARM1, EMSP, PSTS, AI2A1, EMSP1, KLK-L1, PRSS17, Kallikrein, Kallikrein-4, Enamel matrix serine proteinase 1)

Kallikreins (KLKs) belong to the serine protease family of proteolytic enzymes having diverse physiological functions (1). Kallikrein 4 (KLK4), also known as prostase or EMSP1 (enamel matrix serine protease 1), contains one peptidase S1 domain and the expression in prostate is regulated by hormone (1,2). Many kallikreins are implicated in carcinogenesis and have potential as novel cancer and other disease biomarkers (3). KLK4 may have additional roles such as functioning as one of the two major enamel proteases identified that process enamel matrix proteins. Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) (4).

Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry (paraffin). Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Kidney Tissue lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to 15aa near the center of human KLK4.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human KLK4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Stephenson SA, Verity K, Ashworth LK, et al. Localization of a new prostate-specific antigen-related serine protease gene, KLK4, is evidence for an expanded human kallikrein gene family cluster on chromosome 19q13.3-13.4. J. Biol. Chem. 1999; 274:23210-4. 2. Myers SA and Clements JA. Kallikrein 4 (KLK4), a new member of the human kallikrein gene family is up-regulated by estrogen and progesterone in the human endometrial cancer cell line, KLE. J. Clin. Endocrinol. Metab. 2001; 86:2323-6. 3. Kontos CK, Chantzis D, Papadopoulos IN, et al. Kallikrein-related peptidase 4 (KLK4) mRNA predicts short-term relapse in colorectal adenocarcinoma patients. Cancer Lett. 2013; 330:106-12 4. Shimizu-Okabe C, Yousef GM, Diamandis EP, et al. Expression of the kallikrein gene family in normal and Alzheimer’s disease. Neuroreport 2001; 12:27447-51.
USBio References
No references available
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