Technical Data

352349
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu Mo Rt
Accession #
NP_068805
Gene #
LAMTOR3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-LAMTOR3 (MP1, MAPBP, MAPKSP1, PRO0633, MAP2K1IP1, Ragulator3, PRO2783, Ragulator complex protein LAMTOR3, MEK-binding partner 1, Mp1)

The late endosomal/lysosomal adaptor MAPK and MTOR activator 3 (LAMTOR3) protein belongs to the LAMTOR family of proteins, and together with LAMTOR2 and the MAPK1 and ERK kinase 1 (MEK1) localizes to late endosomes where it is required for the efficient activation of ERK signaling (1,2). This complex is involved in the regulation of late endosomal traffic and cellular proliferation (3) and plays a role in cellular host defense against Salmonella infection (4).

Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 20ug/ml Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Brain Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to 15aa near the center of human LAMTOR3.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human LAMTOR3. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Wunderlich W, Fialka I, Teis D, et al. A novel 14-kilodalton protein interacts with the mitogen-activated protein kinase scaffold MP1 on a late endosomal/lysosomal compartment. J. Cell Biol. 2001; 152:765-76. 2. Teis D, Wunderlich W, and Huber LA. Localization of the MP1-MAPK scaffold complex to endosomes is mediated by p14 and required for signal transduction. Dev. Cell 2002; 3:803-14. 3. Teis D, Taub N, Kurzbauer R, et al. p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis. J. Cell Biol. 2006; 175:861-8. 4. Taub N, Nairz M, Hilber D, et al. The late endosomal adaptor p14 is a macrophage host-defense factor against Salmonella infection. J. Cell Sci. 2012; 125:2698-708.
USBio References
No references available
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