Technical Data

352517
Clone Type
Polyclonal
Host
Chicken
Source
Human
Isotype
IgY
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu Mo Rt
Accession #
NP_079440
Shipping Temp
Blue Ice
Storage Temp
-20°C
Chicken Anti-SIK3, CT (L19, QSK, SIK-3, L19, Salt-Inducible Kinase 3)

Salt-inducible kinase family (SIKs) proteins are thought to have a role in steroidogenesis, adipogenesis or regulation of tumor malignancy. Three members (SIK1, SIK2 and SIK3) in the SIK family have been identified thus far. Their kinase domain sequences are closely homologous to those of AMP-activated protein kinase (AMPK). SIK3 can be phosphorylated by a tumor- suppressor kinase LKB1. It is highly and preferentially expressed in ovarian tumors but not in adenomyosis and leiomyoma and may be a potential diagnostic marker for ovarian cancers.

Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Immunofluorescence: 20ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Rat Brain Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 18aa from human SIK3 at C-terminal.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SIK3. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Katoh Y, Takemori H, Horike N, et al. Salt-inducible kinase (SIK) isoforms: their involvement in steroidogenesis and adipogenesis. Mol. Cell. Endocrinol. 2004; 217:109-12. 2. Al-Hakim AK, Goransson O, Deak M, et al. 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK. J. Cell Sci. 2005; 118:5661-73. 3. Charoenfuprasert S, Yang YY, Lee YC, et al. Identification of salt-inducible kinase 3 as a novel tumor antigen associated with tumorigenesis of ovarian cancer. Oncogene 2011; 30:3570-84.
USBio References
No references available
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