Technical Data

352666
Clone Type
Polyclonal
Host
Chicken
Source
Human
Isotype
IgY
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu
Accession #
NP_115916
Shipping Temp
Blue Ice
Storage Temp
-20°C
Chicken Anti-ZGPAT, CT (ZIP, ZC3H9, GPATC6, GPATCH6, Zinc Finger CCCH-Type with G Patch Domain-Containing Protein, G Patch Domain-Containing Protein 6)

The Zinc finger CCCH-type with G patch domain-containing protein (ZGPAT), is a transcriptional repressor that regulates several cellular pathways including EGFR pathways that are involved in cell proliferation, migration and survival. ZGPAT recruits the Mi-2/NuRD complex and represses the expression of EGFR. ZGPAT is down-regulated in breast carcinomas and its expression is negatively correlated with that of EGFR, suggesting that it may be a potential target for breast cancer therapy. Multiple isoforms of ZGPAT are known to exist, including sZIP, which binds to the Mi-2/NuRD complex but lacks the DNA binding component of full-length ZGPAT, thereby antagonizing transcription repression and growth inhibition by ZGPAT.

Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Immunofluorescence: 20ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
SK-N-SH Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 15aa from human ZGPAT at C-terminal.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ZGPAT.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Li R, Zhang H, Yu W, et al. ZIP: a novel transcription repressor, represses EGFR oncogene and suppresses breast carcinogenesis. EMBO J. 2009; 28:2763-6. 2. Yu W, Li R, Gui B, et al. sZIP, an alternative splice variant of ZIP, antagonizes transcription repression and growth inhibition by ZIP. J. Biol. Chem. 2010; 285:14301-7.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net