Technical Data

359720
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-A2M (Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 5, CPAMD5)

alpha-2-Macroglobulin (a-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, a-2M was characterized as a pan-proteinase inhibitor thatwas able to “bait” proteinases into cleaving specific peptide sequences on a-2M. This interaction induces a conformational change in a-2M, thus enabling it to “trap”the proteinase and inhibit its further activity. Subsequently, a2M has also been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor B (TGFB) in serumis primarily bound to a-2M,which renders TGFB inactive. a-2M also binds to IL-6 and, thereby, increasesthe concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved inmediating the inflammatory cascade. Mutations and deletions in the gene encoding a-2M are associated with an increased incidence of Alzheimer’s Disease (AD),which isconsistent with the role of a2M inmediating the clearance and degradation of AB, the major component of B-Amyloid deposits accumulated during AD.

Applications
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human A2M.
Form
Supplied as a liquid in PBS, pH 7.6, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human A2M. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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