Hamster Anti-c-Myc Epitope Tag (Proto-oncogene c-Myc, Transcription Factor p64, Class E Basic Helix-loop-helix Protein 39, BHLHE39, Cmyc) (Chimeric)
Recombinant monoclonal antibody to C-myc Epitope Tag with variable regions (i.e., specificity) from the hybridoma 9E10. Manufactured using a proprietary recombinant platform technology. This is a chimeric hamster antibody, based on the original mouse IgG1 format.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Applications
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: frozen and paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa408-439 from the C-terminal of human c-myc.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% Proclin-300.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the epitope sequence, EQKLISEEDL, located in aa410-419 of human c-myc protein.
