Mouse Anti-ISAV (Infectious Salmon Anemia Virus Nucleoprotein)
ISAV is an enveloped virus that belongs to the family Orthomyxoviridae and genus Isavirus. Its genoma comprises eight negative-sense, single-stranded RNA segments and encodes four major structural proteins including the matrix, the nucleoprotein, and two membrane glycoproteins (hemagglutinin-esterase and gp50). ISAV infects mainly endothelial cells and leukocytes and causes a multisystemic disease characterized by high mortality with exophthalmia, pale gills, ascites, hemorrhagic liver necrosis, and renal interstitial hemorrhage.
Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:2000 ELISA: 1:2000 Western Blot: 1:1000 Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified ISA virus.
Form
Supplied as a liquid in PBS. No preservative added.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes Infectious salmon anemia virus (ISAV).
