Rabbit Anti-ADCY1 (Adenylate Cyclase 1, Brain, AC1, ATP pyrophosphate-lyase 1, Ca(2+)/calmodulin- activated adenylyl cyclase, Adenylate cyclase type I)
Adenylate cyclase type 1 is aform of adenylate cyclase expressed in brain. A similar protein in mouse is involved in pattern formation of the brain. The neural-specific, calmodulin-sensitive adenylyl cyclase (type I), which was first cloned from bovine brain, has been implicated in learning and memory. Villacres et al. (1993) cloned the gene for human fetal brain type I adenylyl cyclase. By database analysis and PCR of human embryonic kidney cell cDNA, Ludwig and Seuwen (2002) cloned full-length ADCY1. The deduced protein contains 1,119aa. Semiquantitative RT-PCR detected ADCY1 highly expressed in peripheral blood leukocytes. Moderate or low ADCY1 levels were detected in brain and most other tissues examined except thymus, prostate, and small intestine.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RILFNL LPAHVAQHFL MSNPRNMDLY YQSYSQVGVM FASIPNFNDF YIELDGNNMG VECLRLLNEI IADFDELMEK DFYKDIEKIK TIGSTYMAAV GLAPTSGTKA KKSISSHLST LADFAIEMFD VLDEINYQSY NDFVLRVGIN VGPVVAGVIG ARRPQYDIWG NTVNVASRMD STGVQGRIQV TEEVHRLLRR CPYHFVCRGK VSVKGKGEML TYFLEGRTDG N
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Arg835-Asn1061 of human ADCY1, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ADCY1.
