Rabbit Anti-ASNS (Asparagine Synthetase, Aspartate-Ammonia Ligase, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase)
Asparagine synthetase is an enzyme that generates asparagine from aspartate. This amidation reaction is similar to that promoted by glutamine synthetase.human ASNS based on its ability to complement ts11, a temperature-sensitive (ts) mutant of the BHK hamster cell line that at the nonpermissive temperature is blocked in progression through the G1 phase of the cell growth cycle. The gene was transcribed into an mRNA of 2 kb that was expressed in all human, hamster, and mouse cell lines tested. It encodes a protein of about 550aa. The 5-prime upstream region of this gene, like other housekeeping genes, lacks conventional TATA and CAAT boxes. Both the human and the hamster genes have a high 5-prime G+C content which may play a role in expression through DNA methylation.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to His213-Tyr536 of human ASNS, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ASNS.
