Technical Data

362825
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DEFa3 (Defensin Alpha 3, Neutrophil Specific, HNP3, DEF3, HNP-2, Neutrophil defensin 2)

DEFA1 and DEFA3 differ from each other only by their N-terminal aa (ala and asp, respectively). DEFA2 is identical to both except that it lacks this N-terminal aa. DEFA1-, DEFA2-, and DEFA3-specific antibodies depleted antiviral activity in a dose-dependent manner, particularly against viruses using CXCR4 rather than CCR5 as a coreceptor. Addition of synthetic or purified natural defensins inhibited HIV-1 replication in vitro. Flow cytometric analysis determined that in addition to neutrophils, a small population of CD8-positive T lymphocytes harbor and secrete DEFA1, DEFA2, and DEFA3. Zhang et al. (2002) proposed that these defensins account for much of the anti-HIV-1 activity of CAF that is not attributable to beta chemokines.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Pro21-Cys94 of human DEFa3, fused to N-terminal His-Tag expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DEFa3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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