Rabbit Anti-DGKa (Diacylglycerol Kinase Alpha, DGK-A, DAGK, DAGK1, DGK-alpha, 80kD diacylglycerol kinase, Diglyceride kinase alpha)
Diacylglycerol kinase alpha is an enzyme belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Diacylglycerol (DAG) functions in intracellular signaling pathways as an allosteric activator of protein kinase C (PKC). In addition, DAG appears to play a role in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to His305-Arg531 of mouse DGKa, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse DGKa.
