Rabbit Anti-GDF15 (Growth DifferentiationFactor 15, PDF, MIC1, NAG-1, PLAB, PTGFB, Macrophage inhibitory cytokine 1, NSAID-activated gene 1 protein, Placental bone morphogenetic protein, Prostate differentiation factor)
GDF15 is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes.GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB.GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. Northern blot analysis showed that MIC1 is not expressed in undifferentiated cells but is progressively upregulated upon differentiation with retinoic acid followed by phorbol ester or cytokine treatment. Exposure of macrophages to MIC1 or TGFB1, followed by stimulation with lipopolysaccharide, suppressed the production of TNFA.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-L SLAEASRASF PGPSELHSED SRFRELRKRY EDLLTRLRAN QSWEDSNTDL VPAPAVRILT PEVRLGSGGH LHLRISRAAL PEGLPEASRL HRALFRLSPT ASRSWDVTRP LRRQLSLARP QAPALHLRLS PPPSQSDQLL AESSSARPQL ELHLRPQAAR GRRRARARNG DHCPLGPGRC CRLHTVRASL EDLGWADWVL SPREVQVTMC IGACPSQFRA ANMHAQIKTS LHRLKPDTVP APCCVPASYN PMVLIQKTDT GVSLQTYDDL LAKDCHCI
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Leu30-Ile308 of human GDF15, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GDF15.
