Technical Data

363402
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GZMH (Granzyme H, CCP-X, CGL-2, CSP-C, CTLA1, CTSGL2, Cathepsin G-Like 2,Protein h-CCPX, Cytotoxic T-lymphocyte proteinase, Cytotoxic serine protease C)

GZMH is located in a cluster on 14q11.2 with cathepsin G and CTSGL1. A similar cluster of genes in the mouse is located on chromosome 14 near the TCRA locus. Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and natural killer cells. Their purpose is to induce apoptosis within virus-infected cells, thus destroying them.Cytotoxic T cells and natural killer cells release a protein called perforin, which attacks the target cells. Researchers used to think that perforin creates pores within the cells, through which the granzymes can enter, inducing apoptosis. However, new evidence indicates that GrB complex can enter a cell through the mannose 6-phosphate receptor and is enclosed in a vesicle.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Glu19-Leu246 of human GZMH, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GZMH.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net