Rabbit Anti-IFNgR1 (Interferon Gamma Receptor 1, CD119)
Interferons may be regarded as polypeptide hormones because of their role in communicating from cell to cell a specific set of instructions that lead to a wide variety of effects. Viruses induce type I interferon, subdivided into alpha-interferon, produced by leukocytes or lymphoblastoid cells, and beta-interferon, produced by fibroblasts. Mitogens and antigenic stimuli induce in lymphocytes type II, immune, or gamma-interferon. The biologic effects of human interferons, including increment of histocompatibility antigens, are mediated through species-specific receptors. Human interferons are not active, for example, in mouse cells. The human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. IFNGR1 is the ligand-binding subunit.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Glu18-Gly245 of human IFNgR1, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IFNgR1.
