Technical Data

364126
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E IC IHC WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MFN1 (Mitofusin 1,Hfzo1, Hfzo2, Transmembrane GTPase MFN1, Fzo homolog)

Mitofusin-1 is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. The deduced 741aa protein contains an N-terminal GTPase domain and a transmembrane domain near the C terminus. MFN1 shares 60% identity with MFN2, with the most extensive homology in the GTPase domains. The N- and C-terminal ends are more variable. When stably expressed in COS-7 cells, MFN1 colocalized with mitochondrial markers. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Met1-Ile234 of mouse MFN1, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse MFN1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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