Rabbit Anti-NIN (Ninein, GSK3B Interacting Protein, Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein)
Ninein is one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. The deduced 2047aa protein has a calculated molecular mass of 239kD. NIN contains an N-terminal GTP-binding site, a large coiled-coil domain with 4 leucine zipper motifs, and a C-terminal domain responsible for GSK binding. Unlike mouse Nin, it does not have an N-terminal Ca(2+)-binding site. Northern blot analysis detected a 9.0-kb transcript in all tissues examined and a 7.0-kb transcript predominantly in heart and skeletal muscle.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Met1-Glu153 of rat NIN, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat NIN.
