Rabbit Anti-PCPE1 (Procollagen C Proteinase Enhancer 1, PCOLCE, PCPE, Procollagen Type 1 COOH-Terminal Proteinase Enhancer, Procollagen C Endopeptidase Enhancer, Type I procollagen COOH-terminal proteinase enhancer)
These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. In the mouse, procollagen C-proteinase depends for full expression upon the presence of either a 55kD glycoprotein or smaller 36- and 34kD proteolytically processed forms of the same protein. None of the 3 enhancer proteins exhibits intrinsic procollagen processing activity, but all are capable of enhancing the activity of the C-proteinase by approximately one order of magnitude. type I procollagen COOH-terminal proteinase enhancer, a glycoprotein that binds to the COOH-terminal propeptide of type I procollagen and enhances C-proteinase activity, from mouse fibroblast culture medium.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GGD VKGESGYVAS EGFPNLYPPN KECIWTITVP EGQTVSLSFR VFDLELHPAC RYDALEVFAG SGTSGQRLGR FCGTFRPAPL VAPGNQVTLR MTTDEGTGGR GFLLWYSGRA TSGTEHQFCG GRLEKAQGTL TTPNWPESDY PPGISCSWHI IAPPDQVIAL TFEKFDLEPD TYCRYDSVSV FNGAVSDDSR RLGKFCGDAV PGSISSEGNE LLVQFVSDLS VTADGFSASY KTLPRGTAKE GQGPGPKRGT EPKVKLPPKS QPPEKTEESP SAPDAPTCPK QCRRTGTLQS NFCASSLVVT ATVKSMVREP GEGLAVTVSL IGAYKTGGLD LPSPPTGASL KFYVPCKQCP PMKKGVSYLL MGQVEENRGP VLPPESFVVL HRPNQDQILT NLSKRKCPSQ PVRAAASQD
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Gly38-Asp449 of human PCPE1, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PCPE1.
