Rabbit Anti-PRDX6 (Peroxiredoxin 6, 1-Cys, AOP2, NSGPx, PRX, aiPLA2, p29, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, Non-selenium glutathione peroxidase, Red blood cells page spot 12)
Peroxiredoxin-6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Kim et al. (1997) isolated a calcium-independent lysosomal PLA2 from a human myeloblast cell line. The predicted 224aa protein contained the PLA2 catalytic motif, but no other significant homology to known phospholipases. Expressed protein had significant PLA2 activity on several substrates.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa1-224 of bovine PRDX6, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes bovine PRDX6. Species Crossreactivity: human.
