Rabbit Anti-SFRP4 (Secreted Frizzled Related Protein 4, FRP-4, FRPHE, Frizzled protein, human endometrium)
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. Sequence analysis predicted that the 346aa SFRP4 protein contains an N-terminal signal peptide, no transmembrane domain, and a hydrophilic C terminus. In situ hybridization analysis demonstrated exclusive expression in stromal and myometrial cells, particularly in endometrium and breast. Upregulated expression was detected in endometrial and breast carcinomas.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.2-2ug/ml Immunocytochemistry: 5-20ug/ml Immunohistochemistry (frozen/paraffin): 5-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Ala22-Val346 of human SFRP4, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human SFRP4.
