Rabbit Anti-SHC1 (SHC-Transforming Protein 1, SHC, SHCA, p52SHC, p66, p66SHC, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1)
The SHC gene encodes a signaling and transforming protein containing Src homology 2 and 3 (SH2 and SH3) domains. The SHC gene encodes 2 widely expressed overlapping proteins of 46 and 52kD, both containing a C-terminal SH2 domain. Adjacent to the SH2 region is a glycine- and proline-rich region. The 2 proteins differ in their N terminals. SHC proteins are involved in mitogenic signal transduction and act by coupling growth factor receptors to the RAS signaling pathway. The protein encoded by the SHC1 gene is thought to act as an adaptor in many signal transduction pathways, for example, facilitating the activation of RAS proteins in response to a variety of factors. SHC proteins are rapidly associated with and phosphorylated by growth factor receptors with intrinsic tyrosine kinase activity.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-2ug/ml Immunocytochemistry: 5-20ug/ml Immunohistochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa272-559 of human SHC1, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human SHC1.
