Rabbit Anti-SRPK2 (SRSF Protein Kinase 2, SFRSK2, SFRS Protein Kinase 2, Serine/arginine-rich protein-specific kinase 2)
Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. This process requires small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP splicing factors, both of which are stored in nuclear 'speckles.' Non-snRNP factors containing an arginine/serine-rich (RS) domain, designated 'SR proteins', are phosphorylated, predominantly on serines within their RS domains. The predicted Srpk2 protein contains conserved serine/threonine kinase domains, a proline-rich domain, a basic region which may be a nuclear localization signal, and an acidic domain. The mouse Srpk2 protein is 58% and 32% identical to the human SRPK1 and yeast Dsk1 proteins, respectively. The authors demonstrated that Srpk2 phosphorylated ASF/SF2 in vitro.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- E LANIDPTWIE SPKTNGHIEN GPFSLEQQLD DEDDDEEDCP NPEEYNLDEP NAESDYTYSS SYEQFNGELP NGRHKIPESQ FPEFSTSLFS GSLEPVACGS VLSEGSPLTE QEESSPSHDR SRTVSASSTG DLPKAKTRAA DLLVNPLDPR NADKIRVKIA DLGNACWVHK HFTEDIQTRQ YRSIEVLIGA GYSTPADIWS TACMAFELAT GDYLFEPHSG EDYSRDEDHI AHIIELL
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Glu380-Leu617 of human SRPK2, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SRPK2.
