Rabbit Anti-TMEM1 (Transmembrane Protein 1, EHOC1, TRAPPC10,TRS130, Trafficking Protein Particle Complex Subunit 10, Epilepsy holoprosencephaly candidate 1 protein, Transport protein particle subunit TMEM1)
TMEM1is a transmembrane protein found in the cis-Golgi complex. The encoded protein is part of the multisubunit transport protein particle (TRAPP) complex and may be involved in vesicular transport from the endoplasmic reticulum to the Golgi. Mutations in this gene could be responsible for the Unverricht-Lundborg type of progressive myoclonus epilepsy, or for autoimmune polyglandular disease type 1. This gene mapped less than 45 kb centromeric of D21S25 and spanned at least 56 kb of genomic DNA. Northern blot analysis revealed that 8-, 7.5-, and 5.3-kb transcripts are ubiquitously expressed in adult tissues. DNA sequence analysis showed a complete coding sequence of 3,570 bp with multiple putative transmembrane domains that shared partial homology with transmembrane proteins including sodium channel proteins.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Pro74-Trp357 of human TMEM1, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TMEM1.
