Rabbit Anti-YWHAq (Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Theta, HS1, 14-3-3, 1C5, Protein HS1, 14-3-3 protein T-cell, 14-3-3 protein tau)
YWHAq product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.The deduced 245aa protein has a calculated molecular mass of 28kD and shares 72% aa identity with bovine brain 14-3-3-eta. Northern blot analysis of Jurkat and HeLa cells showed strong bands at 1.9 and 2.1 kb, and a weaker band at 1.7 kb.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Met1-Glu241 of human YWHAq, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human YWHAq.
