Technical Data

366947
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
N6-P2H5*G8
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu
Accession #
NP_000777
Gene ID
1595
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CYP51A1 (Lanosterol 14-alpha Demethylase, CYP51A1, CYPL1, CYPLI, Cytochrome P450 51A1, Cytochrome P450, Family 51, Subfamily A, Polypeptide 1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450L1, Cytochrome P450LI, LDM, Sterol 14-alpha Demethylase)

Lanosterol 14-alpha demethylase (EC 1.14.13.70; UniProt Q16850; also known as CYP51A1, CYPL1, CYPLI, Cytochrome P450 51A1, Cytochrome P450, family 51, subfamily A, polypeptide 1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450L1, Cytochrome P450LI, LDM, Sterol 14-alpha demethylase) is encoded by the CYP51A1 (also known as CYP51) gene (Gene ID 1595) in human. Cytochromes P450 (CYP) proteins are primarily membrane-associated oxidases located either in the inner membrane of mitochondria or in the endoplasmic reticulum where they function as the terminal enzymes in electron transfer chains. In addition to processing endogenous substrates, CYPs also function to metabolize exogenous drugs and potentially toxic chemicals. The human CYP superfamily consisits of 57 genes and more than 59 pseudogenes divided into 18 families and 43 subfamilies. CYP51A1 represents the only member of the CYP51 subfamily and is involved in the conversion of lanosterol to 4,4-dimethylcholesta-8(9),14,24-trien-3 -ol. Products generated by the CYP51 reaction are vital intermediates in pathways leading to the formation of cholesterol in humans, ergosterol in fungi, and other types of sterols in plants. Sterols play an important structural role in the regulation of membrane fluidity and permeability, which influence the functions of enzymes, ion channels, and other cell components embedded within.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:125 detects CYP51A1 in 50ug of human liver microsome lysate. Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Ovalbumin-conjugated linear peptide corresponging to a sequence near the C-terminus of human/mouse/rat CYP51A1. Species sequence homology: mouse and rat.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CYP51A1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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