Mouse Anti-CD11B (Integrin alpha-M, CD11 Antigen-like Family Member B, CR-3 alpha Chain, Cell Surface Glycoprotein MAC-1 Subunit alpha, Leukocyte Adhesion Receptor MO1, Neutrophil Adherence Receptor, CD11b, ITGAM, CR3A, Integrin alphaM, CR-3alpha, CR3alpha)
CD11b is a 165kD adhesion glycoprotein that associates with the 95kD integrin beta2 (CD18) to form the CD11b/CD18 complex, also known as Mac-1 or CR3. CD11b is expressed on activated lymphocytes, monocytes, granulocytes, and a subset of NK cells. CD11b functions in cell-cell and cell-substrate interactions and is a receptor for iC3b, CD54 (ICAM-1), CD102 (ICAM-2) and CD50 (ICAM-3). This antibody is directed against the CD11b antigen on human monocytes and granulocytes.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume followed by a secondary florescent conjugated anti-mouse antibody Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity
Recognizes human CD11B.
