367048
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
APCIsotype
IgG1,kClone Number
OKT11Grade
Highly PurifiedApplications
FCCrossreactivity
HuAccession #
NM_001767.3Gene ID
914Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeMouse Anti-CD2 (T-cell Surface Antigen CD2, Erythrocyte Receptor, LFA-2, LFA-3 Receptor, Rosette Receptor, T-cell Surface Antigen T11/Leu-5, SRBC) (APC)
CD2 is a 50kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is a specific marker for T lymphocytes, natural killer cells, thymic B cell subsets, and some antigen presenting cells. CD2 interacts with CD58 (LFA-3) and CD48 to mediate cell adhesion and induce co-stimulatory signals in T lymphocytes.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Human acute lymphoblastic leukemia cells
Form
Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC)
Specificity
Recognizes human CD2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Berney S.M, et al. 2000. J Investig Med. 48:102. 2. Ip S.H, et al. 1982. Clin Chem. 28:1905. 3. Ip S.H, et al. 1982. Blood. 60:795. 4. Moingeon P, et al. 1989. Immunol Rev. 111:111. 5. Reinherz E.L, et al. 1989 Cold Spring Harb Symp Quant Biol. 54:611. 6. Springer T.A, et al. 1987. Annu Rev Immunol. 5:223.|7. Verbi W, et al. 1982. Eur J Immunol. 12:81.USBio References
No references available