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367174 Mouse Anti-IgG2a,k Mouse Isotype Control

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG2a,k
Clone Number
RPC5.4
Grade
Affinity Purified
Applications
FC IC IHC IP
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

367174 is a monoclonal immunoglobulin useful isotype control (negative control) for a comparative expression analysis including flow cytometry, immunoprecipitation, immunohistochemistry and immunocytochemistry in both human and mouse experimental models.

Applications
Suitable for use in a comparative expression analysis including Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry in both human and mouse experimental settings. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Application Information
Each lot of purified RPC5.4 has been routinely validated with four serially diluted concentrations to determine any non-specific binding in human PBMCs by flow cytometric analysis. It is suggested to use the same concentration of purified RPC5.4 isotype control immunoglobulin as that of IgG2a, k primary antibody.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide
Purity
Purified by Protein A affinity chromatography.
References
1. Ohno S, et al. 1977. J Natl Cancer Inst. 58:229. 2. Ohno S, et al. 1975. J Natl Cancer Inst. 55:569. 3. Percy ME, et al. 1976. Can J Biochem. 54:675.
USBio References
No references available
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