Technical Data

368510
Clone Type
Monoclonal
Host
Human
Isotype
IgG1,k
Clone Number
ZKA64
Grade
Purified
Applications
N
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Human Anti-Zika Virus Envelope (EIII Domain) (ZIKV E Protein)

The main route of Zika Virus (ZIKV) infection is through bites by Aedes mosquitos, but the virus may also be sexually and vertically transmitted. Although most of the ZIKV infections are asymptomatic or cause only mild symptoms, there is evidence that ZIKV infection can lead to neurological complications, such as Guillain-Barre syndrome in adults and congenital birth defects, including microcephaly in the developing fetus, likely through its ability to infect human neural progenitor cells. Whereas flavivirus envelope (E) proteins mediate fusion and are the main target of neutralizing antibodies, the non-structural protein 1 (NS1) is secreted by infected cells and is involved in immune evasion and pathogenesis. The E protein contains three domains: EDI involved in the conformational changes required for viral entry; EDII containing the fusion loop; and EDIII which may be involved in binding to cellular receptors. The best specific neutralizing antibodies to ZIKV are directed against the domain EDIII of ZIKV.

Applications
Suitable for use in Neutralization. Other applications not tested.
Recommended Dilutions
Neutralization: Neutralizes Zika Virus (ZIKV) with an IC50 of ~93ng/ml. Also enhances ZIKV infection in non-permissive K562 cells at a broad range of concentrations (not above 1ug/ml). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
EBV-immortalized memory B cells derived from ZIKV-infected, DENV-naive human donors.
Form
Supplied as a liquid in PBS. No preservative added.
Purity
≥95% (SDS-PAGE). Endotoxin: ≤0.001EU/ug.
Specificity
Recognizes and binds specifically to the EDIII domain of the Zika Virus envelope protein (ZIKV E). It does not cross-react with Dengue virus envelope protein (DENV E).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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