Mouse Anti-FOXP3 (Forkhead Box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER)
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein fragment corresponding to aa297-431of human FOXP3 expressed in E. coli.
Form
Supplied as a liquid in PBS, 0.05% sodium azide
Specificity
Recognizes human FOXP3.