Technical Data

368880
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
7G8A4
Grade
Purified
Applications
E FC IC IHC WB
Crossreactivity
Hu
Gene ID
5872
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Rab13 (Member RAS Oncogene Family, GIG4)

This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12.

Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Immunocytochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein fragment corresponding to aa66-200 of human Rab13 expressed in E. coli.
Form
Supplied as a liquid in PBS, 0.05% sodium azide
Purity
Purified
Specificity
Recognizes human Rab13.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. J Histochem Cytochem. 2012 Jul; 60(7):537-49. 2. J Cell Biol. 2008 Sep 8; 182(5):845-53.
USBio References
No references available
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