Technical Data

368895
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
5A8E5
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Gene ID
10111
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-RAD50 (DNA Repair Protein RAD50, NBSLD, RAD502, hRad50)

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein fragment corresponding to aa228-359 of human RAD50 expressed in E. coli.
Form
Supplied as a liquid in PBS, 0.05% sodium azide
Purity
Purified
Specificity
Recognizes human RAD50.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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