Rabbit Anti-ACOX1 (Acyl Coenzyme A Oxidase 1, Palmitoyl, ACOX, PALMCOX, SCOX, Peroxisomal Acyl-Coenzyme A Oxidase 1, Straight-chain acyl-CoA oxidase)
Peroxisomal acyl-coenzyme A oxidase 1 is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified.The deduced human protein sequence is 89% similar to the rat protein. Like the rat homolog, the human sequence also contains C-terminal ser-lys-leu (SKL) residues which function as a peroxisomal targeting signal. Two mRNA species, 1.9-kb and 2.1-kb, were identified in liver.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ACOX1 (Arg210~Asn409)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ACOX1.