Technical Data

377283
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo Rt
Gene ID
12501
Gene #
Cd3e
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CD3 epsilon
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, Cd3e

CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.1-0.5ug/ml Western Blot: 0.1-0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to mouse CD3 epsilon (Position: D22-D108) expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse CD3 epsilon. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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