378043
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG,kClone Number
10E10Grade
Affinity PurifiedApplications
IC IP WBCrossreactivity
HuAccession #
NP_002559.2Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-Poly(A)-binding Protein 1 (PABP1, PABP-1, PBP1, PAB1, PABP, PABPC1, PABPC2, PABPL1, Poly(A)-binding Protein Cytoplasmic 1, Polyadenylate Binding Protein 1)
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5ug/ml detected PABP1 in 10ug of HeLa cell lysate. Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human Poly(A)-binding Protein 1.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Poly(A)-binding Protein 1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Gorlach, M., et al., Exp. Cell Res., 211, 400-407 (1994). 2. Adam, S.A., et al., Mol. Cell. Biol., 6, 2932-2943 (1986). 3. Grange, T.C.M., et al., Nuc. Acid Res., 15, 4771-4787 (1987). 4. Craig., A.W.B., et al., Nature, 392, 520-523 (1998).USBio References
No references available