Mouse Anti-Bassoon
Bassoon is a 420kD protein that localizes at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 1:500 - 1:1000 Immunoprecipitation: 2.5ug Immunocytochemistry: 1:400 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to rat Bassoon.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat Bassoon. Species Crossreactivity: human, mouse, rat, bovine and canine