Technical Data

381584
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
17J24
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Gene ID
842
Gene #
CASP9
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Caspase-9 (APAF 3, APAF-3, APAF3, Apoptosis Related Cysteine Peptidase, Apoptotic Protease Activating Factor 3, Apoptotic Protease Mch 6, Apoptotic Protease Mch-6, Apoptotic Protease-Activating Factor 3, CASP 9, CASP-9, CASP9, CASP9_HUMAN, Caspase 9 Apoptosis Related Cysteine Peptidase, Caspase 9 Dominant Negative, Caspase 9c, Caspase-9, Caspase-9 Subunit p10, Caspase9, ICE LAP6, ICE like Apoptotic Protease 6, ICE-LAP6, ICE-like Apoptotic Protease 6, MCH6, PPP1R56, Protein Phosphatase 1, Regulatory Subunit 56, RNCASP)
MCH6

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Caspase-9.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes Caspase-9. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Duan H.,et al.J. Biol. Chem. 271:16720-16724(1996). 2. Srinivasula S.M.,et al J. Biol. Chem. 271:27099-27106(1996). 3. Hadano S., et al.Mamm. Genome 10:757-760(1999). 4. Srinivasula S.M.,et al.Cancer Res. 59:999-1002(1999),|5. Izawa M., et al. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
USBio References
No references available
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