Technical Data

381667
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
17J99
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo
Gene ID
4869
Gene #
NPM1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-NPM1 (B23, NMP1, NO38, NPM 1, NPM, NPM HUMAN, NPM1, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Nucleophosmin (Nucleolar Phosphoprotein B23 Numatrin), Nucleophosmin, Nucleophosmin/B2 3.2, Nucleophosmin/Nucleoplasmin Family Member 1, Nucleoplasmin Family Member 1, Numatrin, TRK Fused Gene)

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to NPM1.
Form
Supplied as a liquid in 0.1M Tris-HCl, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes NPM1. Species Crossreactivity: human, mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Chan W.-Y.,et al.Biochemistry 28:1033-1039(1989).|2. Li X.,et al.Biochem. Biophys. Res. Commun. 163:72-78(1989).|3. Zhang X.T.,et al.Biochem. Biophys. Res. Commun. 164:176-184(1989).|4. Chan P.-K.,et al.Nucleic Acids Res. 25:1225-1232(1997).|5. Okuwaki M.,et al.Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
USBio References
No references available
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