Mouse Anti-p27Kip1 (Cdki1b, CDKN 1B, CDKN 4, CDKN1B, CDKN1B, CDKN4, CDKN4, CDN1B_HUMAN, Cyclin Dependent Kinase Inhibitor 1B, Cyclin Dependent Kinase Inhibitor 1B, Cyclin Dependent Kinase Inhibitor p27, Cyclin Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1), Cyclin-Dependent Kinase Inhibitor 1B, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinase Inhibitor p27 Kip1, KIP 1, KIP1, MEN1B, MEN4, p27, p27 Kip1, P27-like cyclin-Dependent Kinase Inhibitor, P27KIP1)
KIP1
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human p27Kip1.
Form
Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human p27Kip1. Species Crossreactivity: mouse
