Mouse Anti-GRP170 (ORP150, HSP12A, Hypoxia Up Regulated 1, Orp150, Glucose Regulated 170, 150kD Oxygen Regulated)
GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).
Applications
Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunocytochemistry/Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human GRP170.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human GRP170 at ~170kD. Species Crossreactivity: mouse, rat.
