Mouse Anti-JIP2 (IB2, MAPK8IP2, C Jun Amino Terminal Kinase Interacting Protein 2, C-Jun-Amino-Terminal Kinase-Interacting Protein 2, Homologous To Mouse JIP 1, IB 2, IB-2, IB2, Islet Brain 2, Islet-Brain-2, JIP 2, JIP-2, JIP2, JNK Interacting Protein 2, JNK MAP Kinase Scaffold Protein 2, JNK MAP Kinase Scaffold Protein JIP2, JNK-Interacting Protein 2, MAPK8IP2, Mitogen Activated Protein Kinase 8 Interacting Protein 2, Mitogen-Activated Protein Kinase 8-Interacting Protein 2, PRKM8 Interacting Protein Like, PRKM8IPL)
The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. It is expressed mainly in the cerebellum, pituitary gland, occipital lobe, and the amygdala of the brain, but also in the pancreas, including insulin-secreting cells.
Applications
Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunocytochemistry/Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein corresponding to aa226-421 of mouse JIP2. Species sequence homology: human (aa173/200) (86%), rat (aa127/135) (94%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse JIP2 at ~100kD. Does not cross-react with JIP-1/IB-1. Species Crossreactivity: human, rat.
