Mouse Anti-CSF1R (CD115, CSF-1R)
CSF1R (CD115) is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R. CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages. The development of the monoclonal antibody specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:25-1:200 Optimal dilutions to be determined by the researcher.
Hybridoma
Ba/F3 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant fusion protein from porcine CSF1R.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes porcine CSF1R. Species Crossreactivity: canine. Does not react with human, equine, sheep, bovine.
