Rabbit Anti-COX1 (Prostaglandin G/H Synthase 1, Cyclooxygenase-1)
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants.
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:100-1:300 ELISA: 1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa550-599 from human Cox1.
Form
Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes endogenous levels of human Cox1. Species Crossreactivity: mouse and rat
