Rabbit Anti-SIGIRR (Single Ig IL-1-related Receptor, Single Ig IL-1R-related Molecule, Single Immunoglobulin Domain-containing IL1R-related Protein, Toll/interleukin-1 Receptor 8, TIR8, SIGIRR, UNQ301/PRO342)
Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:5000 Immunohistochemistry (Paraffin): 1:20-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa1-120 of human SIGIRR.
Form
Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes human SIGIRR. Species Crossreactivity: mouse
